Gabriel Monteiro

Gabriel A. Monteiro

associate professor

A-2036-2008

Web of Science profile

iBB Group: berg

gabmonteiro@tecnico.ulisboa.pt

Expertise: plasmids, minicircles, DNA vaccines, recombinant proteins, biocementation

Short Bio:

I earned a Bachelor's in Biology from the University of Coimbra, followed by a Master's and Ph.D. in Biotechnology from Instituto Superior Técnico, Lisbon. Currently, I am an associate professor and coordinator of the Biological Engineering master's program at IST. My career has predominantly focused on designing and producing plasmid biopharmaceuticals, and more recently in the field of biocementation. I co-authored 102 articles in peer-reviewed international journals, with over 4600 citations (h-index 35 Google Scholar; h=28 Scopus). As an academic mentor, I have (co)supervised 15 Ph.D. and 37 M.Sc. theses.

Research Focus:

I specialize in the design and production of plasmid and minicircle biopharmaceuticals for application as DNA vaccines and gene therapy vectors for transient cell modification.

Lactic acid bacteria as cell factories: a Synthetic Biology approach for plasmid DNA and recombinant protein production (PTDC/BTM-SAL/28624/2017) (Responsible), finished
https://app.dimensions.ai/details/grant/grant.9566977

AMELIE – Anchored Muscle cELls for IncontinencE”, Horizon 2020, Grant agreement ID: 874807) (team member)
https://cordis.europa.eu/project/id/874807

Quality by design-centered platform for the manufacturing of CAR-engineered NK cells and extracellular vesicles for immunotherapy (PTDC/EQU-EQU/3708/20219) (team member)
https://app.dimensions.ai/details/grant/grant.9953549

Fixation mechanisms and durability of biocementation treatment of soils. Application to a real slope to prevent ravine development (PTDC/ECI-EGC/1086/2021) (team member)
https://app.dimensions.ai/details/grant/grant.9951237

Duarte, S. O. D., Martins, M. C., Andrade, S. M., Prazeres, D. M. F., Monteiro, G. A. (2019). Plasmid copy number of pTRKH3 in Lactococcus lactis is increased by modification of the repDE ribosome binding site. Biotechnol. J. 14, 1800587. https://doi.org/10.1002/biot.201800587

Cardoso, R., Pereira, R., Duarte, S. O. D., Monteiro, G. A. (2020). About calcium carbonate precipitation on sand biocementation. Eng. Geol. 271, 105612. https://doi.org/10.1016/j.enggeo.2020.105612

Silva, M., Monteiro, G. A., Fialho, A. M., Bernardes, N., da Silva, C. L. (2020). Conditioned medium from azurin-expressing human mesenchymal stromal cells demonstrates anti-tumor activity against breast and lung cancer cell lines. Front. Cell Dev. Biol. 8:471. https://doi.org/10.3389/fcell.2020.00471

Serra, J., Alves, C. P. A., Cabral, J. M. S, Monteiro, G. A., da Silva, C. L., Prazeres, D. M. F., (2021). Minicircle-based expression of vascular endothelial growth factor (VEGF) in mesenchymal stromal cells (MSC) from diverse human tissues. J. Gene Med. 23, e3342. https://doi.org/10.1002/jgm.3342

Alves, C. P. A., Prazeres, D. M. F., Monteiro, G. A. (2021). Recombination efficiency measurement by real-time PCR: a strategy to evaluate ParA-mediated minicircle production. Anal. Biochem. 628, 114285. https://doi.org/10.1016/j.ab.2021.114285

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Gabriel A. Monteiro