Nuno Bernardes

assistant researcher

F-7826-2014

iBB Group: bsrg

nuno.bernardes@tecnico.ulisboa.pt

Expertise: Therapeutic peptides, Nanomedicines/Extracellular vesicles, Tumor Microenvironment, Gene Editing, Cellular Therapies

Short Bio:

Nuno Bernardes is a Researcher in Biosciences and Bioengineering. Nuno holds a MSc in Biological Engineering (2008) and a PhD in Biotechnology (2013). His work is focused on: i) protein- and peptide-based strategies to enhance tumor-targeted delivery of nanosized drug delivery systems, and (ii) engineered cell-based therapies for cancer with clinically relevant therapeutic cells. Currently, Nuno is exploring different technical approaches and 3D cellular models to increase therapeutic efficacies by studying peptide-cell interactions in the tumor microenvironment which can improve the release of cargoes from the carriers and modulate the communication of cancer cells with their microenvironment.

Research Focus:

Cancer cell signaling and drug resistanceNanomedicine for cancer therapy: peptide-mediated delivery of nanoparticles and extracellular vesicles Genome engineering: CRISPR/Cas9 Cell-based therapy of cancer

ESCAPE: Mapping the uptake and intracellular processing of p28 and p28-decorated extracellular vesicles to boost the functional transfer of therapeutic RNAs (iBB/2022). internal grants iBB.

Uso de (nano)partículas funcionalizadas para o enriquecimento e a detecção de SARS-CoV-2 em amostras clínicas e ambientais (SAICTCOVID/72536/2020)(member of the team, PI: Dr Leonilde Moreira. Funding Agency: Portugal 2020.

p28Nano: Cell penetrating p28 peptide-mediated delivery of nanomedicines for cancer treatment. iBB/IST and INEB/i3S (PTDC/BTM-SAL/30034/2017) (member of the team; PI: Dr Arsénio Fialho). Funding Agency: FCT – Portuguese Foundation for Science and Technology.

de Almeida Fuzeta, M., Goncalves, P. P., Fernandes-Platzgummer, A., Cabral, J. M. S., & Bernardes Nuno and da Silva, C. L. (2022). From Promise to Reality: Bioengineering Strategies to Enhance the Therapeutic Potential of Extracellular Vesicles. Bioengineering-Basel, 9(11). https://doi.org/10.3390/bioengineering9110675

Garizo, A. R., Castro, F., Martins, C., Almeida, A., Dias, T. P., Fernardes, F., Barrias, C. C., Bernardes, N., Fialho, A. M., & Sarmento, B. (2021). p28-functionalized PLGA nanoparticles loaded with gefitinib reduce tumor burden and metastases formation on lung cancer. Journal of Controlled Release , 337, 329–342. https://doi.org/10.1016/J.JCONREL.2021.07.035

de Almeida Fuzeta, M., Bernardes, N., Oliveira Filipa D. and Costa, A. C., Fernandes-Platzgummer, A., Farinha, J. P., Rodrigues, C. A. V, Jung, S., Tseng, R.-J., Milligan, W., Lee, B., Castanho, M. A. R. B., Gaspar, D., Cabral, J. M. S., & da Silva, C. L. (2020). Scalable Production of Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Under Serum-/Xeno-Free Conditions in a Microcarrier-Based Bioreactor Culture System. Frontiers in Cell and Developmental Biology, 8. https://doi.org/10.3389/fcell.2020.553444

Silva, M., Monteiro, G. A., Fialho, A. M., Bernardes, N., & da Silva, C. L. (2020). Conditioned Medium From Azurin-Expressing Human Mesenchymal Stromal Cells Demonstrates Antitumor Activity Against Breast and Lung Cancer Cell Lines. Frontiers in Cell and Developmental Biology, 8. https://doi.org/10.3389/fcell.2020.00471

Bernardes, N., Garizo, A. R., Pinto, S. N., Canico, B., Perdigao, C., Fernandes, F., & Fialho, A. M. (2018). Azurin interaction with the lipid raft components ganglioside GM-1 and caveolin-1 increases membrane fluidity and sensitivity to anti-cancer drugs. Cell Cycle, 17(13), 1649–1666. https://doi.org/10.1080/15384101.2018.1489178

iBB People

Nuno Bernardes