Nuno Bernardes

Research Activities
My research is focused on the development of bacterial proteins and peptides as anticancer agents. In particular studies aiming to elucidate the cellular and molecular effects of azurin from P. aeruginosa on cancer cells. I am also interested in exploiting gene/genome engineering tools for cell-based therapies of cancer.

Selected Publications
Bernardes N, Garizo AR, Pinto SN, Caniço B, Perdigão C, Fernandes F, Fialho AM (2018) Azurin interaction with the lipid raft components ganglioside GM-1 and caveolin-1 increases membrane fluidity and sensitivity to anti-cancer drugs. Cell Cycle. 7(13):1649-1666. doi: 10.1080/15384101.2018.1489178

Peeters K, Van Leemputte F, Fischer B, Bonini BM, Quezada H, Tsytlonok M, Haesen D, Vanthienen W, Bernardes N, Gonzalez-Blas CB, Janssens V, Tompa P, Versées W, Thevelein JM (2017). Fructose-1,6-bisphosphate couples glycolytic flux to activation of Ras. Nat Commun 8(1):922. doi: 10.1038/s41467-017-01019-z.

Bernardes N, Abreu S, Carvalho FA, Fernandes F, Santos NC, Fialho AM (2016). Modulation of membrane properties of lung cancer cells by azurin enhances the sensitivity to EGFR-targeted therapy and decreased β1 integrin-mediated adhesion. Cell Cycle 15(11):1415-24. doi: 10.1080/15384101.2016.1172147.

Bernardes N, Ribeiro AS, Abreu S, Vieira AF, Carreto L, Santos M, Seruca R, Paredes J, Fialho AM (2014). High-throughput molecular profiling of a P-cadherin overexpressing breast cancer model reveals new targets for the anti-cancer bacterial protein azurin. Int J Biochem Cell Biol 50:1-9. doi: 10.1016/j.biocel.2014.01.023.

Bernardes N, Ribeiro AS, Mota B, Matos RG, Arraiano CM, Seruca R, Paredes J and Fialho AM. (2013) The bacterial protein azurin impairs invasion and FAK/Src signaling in P-cadherin-overexpressing breast cancer cell models. PLoS ONE 8(7): e69023. doi:10.1371/journal.pone.0069023

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