Sílvia Henriques

PhD Student

Research Activities

-Molecular mechanisms involved in the adaption and resistance of the yeast Saccharomyces cerevisiae to chemical stress
-Transcriptional regulation in S. cerevisiae
-Heterologous overexpression of eukaryotic proteins in E. coli
-Purification and crystallization of eukaryotic proteins;
-Structural characterization techniques, particularly NMR (Nuclear Magnetic Resonance NMR), X-ray diffraction and FRET (Förster resonance energy transfer);
-Techniques for molecular interaction characterization, such as SPR (Surface Plasmon Resonance), EMSA (Electrophoretic Mobility Shift Assay), ChIP (Chromatin immunoprecipitation) and Co-immunoprecipitation;
-Global analysis techniques of biological systems, having already executed Synthetic Genetic Array (SGA) Analysis experiments; and the susceptibility analysis of the haploid mutants collection (EUROSCARF) in the presence of formic acid;


Selected Publications


Rodrigues RMM, de-Carvalho J, Henriques SF, Mira NP, Sa-Correia I, Ferreira GNM, “Transmission line model analysis of transcription factors binding to oligoduplexes – differentiation of the effect of single nucleotide modifications.” Analyst, 139, 3871 (2014)


de-Carvalho J, Rodrigues RMM, Tomé B, Henriques SF, Mira NP, Sa-Correia I, Ferreira GNM, “Conformational and Mechanical Changes of DNA upon Transcription Factor Binding Detected by a QCM and Transmission Line Model.” Analyst, 139, 1847 (2014)


Mira NP, Henriques SF, Keller G, Teixeira MC, Matos RG, Arraiano CM, Winge DR, Sá-Correia I, ”Identification of a DNA-binding site for the transcription factor Haa1, required for Saccharomyces cerevisiae response to acetic acid stress.” Nucleic Acids Res. 39(16), 6896-907 (2011)



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