Ana Gabriela Gonçalves Neves Gomes

Researcher


Research Activities

My domain of specialization is:
Biotechnology and bioprocess engineering, namely in the development of purification processes for biopharmaceuticals (plasmid DNA and antibodies) based in several chromatographic steps (affinity, HIC, SEC), tangential flow filtration (TFF), aqueous two phase systems (ATPS) and process scale-up. Circular dichroism applied to proteins and DNA. Standard molecular biology techniques.

Selected Publications

Gomes, A. G., Azevedo, A. M., Aires-Barros, M. R. and Prazeres, D. M. F. (2012). Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography, Journal of Separation Science, 35 (22) 3190-3196

Gomes, A. G., Azevedo, A. M., Aires-Barros, M. R. and Prazeres, D. M. F. (2011). Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronate chromatographic beads. Journal of Chromatography A, 1218 (48) 8629–8637.

Azevedo, A. M., Gomes, A. G., Borlido, L., Santos, I. F. S., Prazeres, D. M. F. and Aires-Barros, M. R. (2010). Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography. Journal of Molecular Recognition, 23 (6) 569-576.

Gomes, A. G., Azevedo, A. M., Aires-Barros, M. R. and Prazeres, D. M. F. (2010). Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption. Journal of Chromatography A, 1217 (15) 2262-2266.

Azevedo. A. M., Gomes. A. G., Rosa. P. A. J., Ferreira, I. F., Pisco, A. M. M. O., Prazeres, D. M. F. and Aires-Barros, M. R. (2009). Partitioning of human antibodies in polyethylene glycol – sodium citrate aqueous two-phase systems. Separation and Purification Technology, 65 (1) 14-21.

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