Ana Margarida Azevedo

Research scientist

Research Activities

The main goal of my research activities is to develop alternative downstream processes for the purification of biopharmaceutical biomolecules, including monoclonal antibodies, recombinant proteins and plasmid DNA. A key feature of these novel processes is that they should allow for scalable and integrated purification platforms. Another focus of my research is the development of animal cell factories for the production of monoclonal antibodies based on Chinese hamster ovary cells (or alternatively hybridoma cells).

Selected Publications

Azevedo, A.M., Rosa, P.A.J., Ferreira, I.F., Aires-Barros, M.R., “Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing”, Trends Biotecnhol., 27, 240-247 (2009)

Azevedo, A.M., Gomes, A.G., Rosa, P.A.J, Ferreira, I.F., Pisco, A.M.M.O., Aires-Barros, M.R., “Partitioning of human antibodies in polyethylene glycol - sodium citrate aqueous two-phase systems”, Sep. Purif. Tecnhol., 65, 14-21 (2009)

Azevedo, A.M., Gomes, A.G., Borlido, L., Santos, I.S., Prazeres, D.M.F., Aires-Barros, M.R., “Capture of human monoclonal antibodies from a cell culture supernatant by phenyl boronate chromatography”, J. Mol. Recognit., 23, 569-576 (2010)

Borlido, L., Azevedo, A.M., Roque, A.C.A., Aires-Barros, M.R., “Potential of boronic acid functionalized magnetic particles in the adsorption of human antibodies under mammalian cell culture conditions”, J. Chromatogr. A, 1218, 7821-7827 (2011)

Rosa, P.A.J., Azevedo, A.M., Sommerfeld, S., Bäcker, W., Aires-Barros, M. R., " Continuous purification of antibodies from cell culture supernatant: from concept to process", Biotechnol. J., 8, 352-362 (2013)

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